There were calls for resignations on Thursday this week after it emerged that the minister for Health in the Murcia Region, Manuel Villegas, had been vaccinated against Covid-19 despite apparently not falling into one of the priority categories.
As well as the ministers, several high-ranking officials along with some staff in his offices had also had the jab, it was reported.
Villegas, who announced his resignation on Wednesday, admitted that he had the first injection "last week" as he is a qualified doctor and the ministry has said that only qualified health workers in his team have had it.
The UGT union had reported that some office workers at the regional health service itself had been vaccinated ahead of frontline staff.
The events in Murcia came after last week it emerged several local mayors across Spain had been vaccinated ahead of priority groups in their municipalities.
Four were suspended by the Socialist party this week. The majority claim that they took the vaccine to save a spare dose going to waste.